Journal: Scientific Reports

Article Title: Increased Ca 2+ content of the sarcoplasmic reticulum provides arrhythmogenic trigger source in swimming-induced rat athlete’s heart model

doi: 10.1038/s41598-020-76496-2

Figure Lengend Snippet: Comparison of spontaneous Ca 2+ releases under 4 Hz stimulation frequency between control ( a ) and trained ( b ) rats. The black arrows indicate the electric stimuli, the grey arrow marks an ineffective stimulus. We found larger number of spontaneous Ca 2+ releases in the trained group, compared to control ( c ).

Article Snippet: After blocking in 5% milk-TBS-T, the membranes were immunolabeled with the respective primary antibodies provided by the Calcium Ion Regulation Antibody Sampler Kit (Cell Signalling Technology; Danvers, MA, USA; overnight, at 4 °C; dilutions: anti-PKA C, anti-pPKA C (-α, -β, and -γ when phosphorylated at Thr197): 1:7000, anti-PLN, anti-pPLN (when phosphorylated at Ser16/Thr17): 1:2500, anti-SERCA2: 1:7000).

Techniques: Comparison, Control

Journal: Scientific Reports

Article Title: Increased Ca 2+ content of the sarcoplasmic reticulum provides arrhythmogenic trigger source in swimming-induced rat athlete’s heart model

doi: 10.1038/s41598-020-76496-2

Figure Lengend Snippet: Assessment of Ca 2+ handling on isolated cells. Panel ( a ) shows identical current–voltage relationship of L-type Ca 2+ current between groups. Panel ( b ) illustrates significantly larger inward current as a response of 10 mM caffeine application. Panel ( c , d ) reports larger Ca 2+ transient amplitude in the case of trained rats (red trace) compared to control (black trace). Panel ( e ) indicates faster transient relaxation kinetics in the case of trained animals.

Article Snippet: After blocking in 5% milk-TBS-T, the membranes were immunolabeled with the respective primary antibodies provided by the Calcium Ion Regulation Antibody Sampler Kit (Cell Signalling Technology; Danvers, MA, USA; overnight, at 4 °C; dilutions: anti-PKA C, anti-pPKA C (-α, -β, and -γ when phosphorylated at Thr197): 1:7000, anti-PLN, anti-pPLN (when phosphorylated at Ser16/Thr17): 1:2500, anti-SERCA2: 1:7000).

Techniques: Isolation, Control

Journal: bioRxiv

Article Title: Antiviral Effect of Hyunggaeyungyo-tang on A549 Cells Infected with Human Coronavirus

doi: 10.1101/2021.06.02.446680

Figure Lengend Snippet: Visualization of PKR, pPKR expression

Article Snippet: PKR and pPKR primary antibodies were purchased from Santa Cruz Biotechnology; eIF2α and peIF2α antibodies were obtained from Cell Signaling Technology.

Techniques: Expressing